Table II.
Degree of intermediate DNA methylation (%) in mesenchymal stem cells (MSCs) obtained from either the iliac crest or the femur using different harvest techniques.a
| A, Gene methylation | ||||
|---|---|---|---|---|
|
| ||||
| Gene | Femur (RIA) | Femur (spoon) | Iliac crest (spoon) | Iliac crest (aspirates) |
| COL1A | M: 0.0 | M: 0.0 | M: 25.0 | M: 0.0 |
| LQ: 0.0 | LQ: 0.0 | LQ: 0.0 | LQ: 0.0 | |
| UQ: 30.1 | UQ: 17.6 | UQ: 34.1 | UQ: 01 | |
| CTNNB1 | M: 0.0 | M: 5.5 | M: 9.6 | M: 5.3 |
| LQ: 0.0 | LQ: 0.0 | LQ: 0.0 | LQ: 0.0 | |
| UQ: 80.5 | UQ: 77.7 | UQ: 38.2 | UQ: 14.9 | |
| RUNX2 | M: 11.4 | M: 40.6 | M: 0.0 | M: 0.0 |
| LQ: 0.0 | LQ: 0.0 | LQ: 0.0 | LQ: 0.0 | |
| UQ: 40.3 | UQ: 99.8 | UQ: 6.6 | UQ: 2.6 | |
| SMAD5 | M: 6.5 | M: 48.6 | M: 82.6 | M: 1.6 |
| LQ: 0.0 | LQ: 0.0 | LQ: 31.3 | LQ: 0.0 | |
| UQ: 100.0 | UQ: 100.0 | UQ: 100.0 | UQ: 43.5 | |
| WNT3A | M: 0.0 | M: 0.0 | M: 0.0 | M: 8.6 |
| LQ: 0.0 | LQ: 0.0 | LQ: 0.0 | LQ: 0.0 | |
| UQ: 0.0 | UQ: 54.4 | UQ: 0.0 | UQ: 11.7 | |
| B, Statistical evaluation (intergroup comparison between different MSC sources) | ||||
|---|---|---|---|---|
|
| ||||
| Gene | Femur (RIA) | Femur (spoon) | Iliac crest (spoon) | Iliac crest (aspirates) |
| COL1A | ||||
| CTNNB1 | ||||
| RUNX2 | p(nc)=0.08 vs. aspirates | p(nc)=0.05 vs. aspirates | ||
| p(nc)=0.08 vs. iliac | ||||
| crest (spoon) | ||||
| SMAD5 | p=0.05 (nc) vs. aspirates | |||
| WNT3A | p(nc)=0.05 vs. RIA | |||
a
Gene methylation was measured by a PCR-based assay as indicated in the Materials and methods. Values are presented as median (M), 25% quartile (LQ) and 75% quartile (UQ) (A). Statistical evaluation (intergroup comparison between different MSC sources) is shown in (B). p(nc) indicate non-corrected (nc) local p-values without Bonferroni-Holm correction for multiple comparisons. Not corrected statistical significance can be interpreted as a statistical trend. RIA, Reamer/Irrigator/Aspirator; spoon, obtained using a spoon; aspirates, obtained by fine-needle aspiration.