Table III.
Percentage of hypermethylated DNA (%) in mesenchymal stem cells (MSCs) obtained either from the iliac crest or from the femur using different harvest techniques.a
| A, Gene methylation | ||||
|---|---|---|---|---|
|
| ||||
| Gene | Femur (RIA) | Femur (spoon) | Iliac crest (spoon) | Iliac crest (aspirate) |
| COL1A | M: 0.6 | M: 1.0 | M: 2.0 | M: 0.1 |
| LQ: 0.5 | LQ: 0.7 | LQ: 0.6 | LQ: 0.1 | |
| UQ: 2.5 | UQ: 8.5 | UQ: 2.9 | UQ: 0.7 | |
| CTNNB1 | M: 0.2 | M: 0.1 | M: 2.6 | M: 0.2 |
| LQ: 0.0 | LQ: 0.0 | LQ: 0.9 | LQ: 0.1 | |
| UQ: 1.2 | UQ: 0.3 | UQ: 10.9 | UQ: 0.4 | |
| RUNX2 | M: 2.1 | M: 1.8 | M: 4.3 | M: 0.6 |
| LQ: 0.4 | LQ: 0.4 | LQ: 2.5 | LQ: 0.3 | |
| UQ: 30.4 | UQ: 14.0 | UQ: 19.5 | UQ: 2.2 | |
| SMAD5 | M: 7.9 | M: 0.4 | M: 2.2 | M: 0.3 |
| LQ: 0.0 | LQ: 0.0 | LQ: 0.0 | LQ: 0.2 | |
| UQ: 26.7 | UQ: 40.8 | UQ: 17.0 | UQ: 3.6 | |
| WNT3A | M: 4.9 | M: 1.7 | M: 2.4 | M: 0.4 |
| LQ: 1.1 | LQ: 0.1 | LQ: 0.2 | LQ: 0.2 | |
| UQ: 82.7 | UQ: 25.3 | UQ: 5.4 | UQ: 0.5 | |
| B, Statistical evaluation (intergroup comparison between MSCs obtained from different sources) | ||||
|---|---|---|---|---|
|
| ||||
| Gene | Femur (RIA) | Femur (spoon) | Iliac crest (spoon) | Iliac crest (aspirates) |
| COL1A | p=0.1 vs. iliac | p=0.05 vs. iliac | p=0.05 vs. iliac | |
| crest aspirates | crest aspirates | crest aspirates | ||
| CTNNB1 | ||||
| RUNX2 | p=0.08 vs. iliac | |||
| crest aspirates | ||||
| SMAD5 | ||||
| WNT3A | p=0.04 vs. iliac | |||
| crest aspirates | ||||
a
Gene methylation was measured by a PCR-based assay as indicated in the Materials and methods. Values are presented as median (M), 25% quartile (LQ) and 75% quartile (UQ) (A). Statistical evaluation (intergroup comparison between different MSC sources) is shown in (B). p(nc) indicate non-corrected (nc) local p-values without Bonferroni-Holm correction for multiple comparisons. Not corrected statistical significance may be interpreted as a statistical trend. RIA, Reamer/Irrigator/Aspirator; spoon, obtained using a spoon; aspirates, obtained by fine-needle aspiration.