Figure 6.
Effect of different doses of rLcIL-1β on bacterial loads in immune tissues and blood of large yellow croaker Fish (6 in each group) were i.p.-injected with V. alginolyticus (6.5×104 CFU/g) and received 0.001, 0.01 or 0.1 µg/g of rLcIL-1β at 0.5 hpi, respectively. The control group received an equal volume of PBS. Fish were euthanized at 72 h post treatment of rLcIL-1β. Liver, spleen, kidney, and blood samples were collected. Homogenates and blood were cultured on TCBS agar plates. Colony numbers were normalized to volume (0.1 mL for blood) and tissue weight (0.1 g for liver, spleen and kidney). *: P<0.05 versus PBS-treated group.