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. 2016 Feb 18;37(4):911–920. doi: 10.3892/ijmm.2016.2493

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Reactive oxygen species (ROS) induces interaction of vascular endothelial growth factor receptor-2 (VEGFR-2) and β-transduction repeat-containing protein (β-TrCP), and suppresses β-TrCP-ubiquitin proteasome system-induced VEGFR-2 upon glucose oxidase (GO) exposure. (A) Intracellular locations of VEGFR-2 and β-TrCP were detected by confocal immunofluorescence microscopy. Green, VEGFR-2; red, β-TrCP; blue, nuclear staining with DAPI. Yellow arrows in Merge panels indicate co-localization of VEGFR-2 and β-TrCP. (B) Immunoprecipitates of anti-VEGFR-2 antibody against human umbilical vein endothelial cell (HUVEC) lysates were resolved and blotted against anti-ubiquitin antibody. Quantification of ubiquitin/input. (C) β-TrCP siRNA suppressed β-TrCP expression but enhanced VEGFR-2 expression in HUVECs exposed to GO. (D) VEGFR-2 protein expression in HUVECs cultured with GO after treatment with 10 µM MG132 (4 h) was increased compared with GO treatment alone. The results are expressed as the means ± SD. ^*P<0.05 vs. normal glucose (NG) group.

Reactive oxygen species (ROS) induces interaction of vascular endothelial growth factor receptor-2 (VEGFR-2) and β-transduction repeat-containing protein (β-TrCP), and suppresses β-TrCP-ubiquitin proteasome system-induced VEGFR-2 upon glucose oxidase (GO) exposure. (A) Intracellular locations of VEGFR-2 and β-TrCP were detected by confocal immunofluorescence microscopy. Green, VEGFR-2; red, β-TrCP; blue, nuclear staining with DAPI. Yellow arrows in Merge panels indicate co-localization of VEGFR-2 and β-TrCP. (B) Immunoprecipitates of anti-VEGFR-2 antibody against human umbilical vein endothelial cell (HUVEC) lysates were resolved and blotted against anti-ubiquitin antibody. Quantification of ubiquitin/input. (C) β-TrCP siRNA suppressed β-TrCP expression but enhanced VEGFR-2 expression in HUVECs exposed to GO. (D) VEGFR-2 protein expression in HUVECs cultured with GO after treatment with 10 µM MG132 (4 h) was increased compared with GO treatment alone. The results are expressed as the means ± SD. ^*P<0.05 vs. normal glucose (NG) group.