FIG. 1.

Production of IFN is reduced in the presence of HSV-1. (A) Total RNA was isolated from infected HEC-1-B cells at 5 hpi with SeV alone (lanes 2 and 6), HSV-1 alone (lanes 3 and 7), or a combination of the two viruses (lanes 4 and 8). Infections were carried out in the presence (lanes 5 to 8) or absence (lanes 1 to 4) of CHX added at the time of infection. The harvested RNA was analyzed by RNase protection assay for the presence of IFN-β mRNA and the control GAPDH mRNA. (B) IFN-β levels in media from the same infected cells were determined by ELISA as described in Materials and Methods. Amounts of secreted IFN-β were not determined for the cells infected in the presence of CHX due to the fact that no protein expression was expected to take place. The data shown are from one experiment representative of several replicate experiments.