Figure 5. DMS- and DLS-Projecting SNc DA Neurons Respond Similarly to Appetitive but Differentially to Aversive Stimulation.

(A) CAV-cre was injected into either the DMS or DLS. AAV-expressing cre-dependent GCaMP6f was injected into the SNc. A 400 µm fiber optic implant was placed in the SNc to allow for measurement of GCaMP6f signals.

(B) Fiber photometry rig schematic. See Experimental Procedures for a more detailed description.

(C) Example of responses to reward observed in a mouse expressing GCaMP6f in DMS-projecting SNc DA neurons. Time 0 is aligned to either rewarded or non-rewarded port entries in an operant chamber. Arrow is placed at time 0. Area of light shading is SEM.

(D) Example of responses to reward observed in a mouse expressing GCaMP6f in DLS-projecting SNc DA neurons.

(E) Quantification of the peak ΔF/F observed in response to reward in mice expressing GCaMP6f in DMS-projecting SNc DA neurons or in mice expressing GCaMP6f in DLS-projecting SNc DA neurons.

(F) Quantification of the ΔF/F observed at time 0 in response to a non-rewarded port entry in mice expressing GCaMP6f in DMS-projecting SNc DA neurons or in mice expressing GCaMP6f in DLS-projecting SNc DA neurons.

(G) Example of responses to shock observed in a mouse expressing GCaMP6f in DMS-projecting SNc DA neurons. Yellow bar indicates the time of the shock (0–0.5 s). Area of light shading is SEM. (H) Example of responses to shock observed in a mouse expressing GCaMP6f in DLS-projecting SNc DA neurons.

(I) Quantification of the peak (extreme, whether negative or positive) ΔF/F observed during the shock in mice expressing GCaMP6f in DMS-projecting SNc DA neurons or in mice expressing GCaMP6f in DLS-projecting SNc DA neurons. ****p < 0.0001.

(J) Quantification of the mean ΔF/F observed 1–5 s post-shock in mice expressing GCaMP6f in DMS-projecting SNc DA neurons or in mice expressing GCaMP6f in DLS-projecting SNc DA neurons. ***p < 0.001. The same mice are shown in (C)–(J). See also Figure S4.