Skip to main content
. Author manuscript; available in PMC: 2016 Mar 14.
Published in final edited form as: J Mater Chem B. 2015 Sep 30;4(9):1672–1685. doi: 10.1039/C5TB01754H

Fig. 3.

Fig. 3

Quantification and optimization of microparticle (MP) to dendritic cell (DC) ratio and immunoarray island spacing. (a) Dendritic cells were cultured with rhodamine-encapsulated microparticles at varying ratios of MPs : DCs and the percentage of MPs phagocytosed was quantified. Dendritic cells were shown to phagocytose ∼90% of the printed particles within the first 24 h when the particle to DC ratio was maintained at 10: 1. A linear relationship was observed between the percent of particles phagocytosed and the MP:DC ratio. (b) Inter-island spacing was investigated to limit paracrine signaling. Poly I:C encapsulated MPs were printed with a range of MP:DC ratios. IL-12p40 production was quantified between immunoarrays printed in randomized configurations at either 1000 μm or 1200 μm center-to-center inter-island distances. Production of IL-12p40 at the 1000 μm spacing (b, left panel) does not provide the dose response fidelity seen with the 1200 μm spacing (b, right panel) suggesting negligible cross-talk between neighboring islands at the larger inter-island distance. (*: p < 0.05).