Skip to main content
. 2016 Mar 14;11(3):e0151087. doi: 10.1371/journal.pone.0151087

Fig 6. SDS-PAGE analyses of 8xHis-StrepII-MBP-3607 purification (A) and tobacco etch virus (TEV) protease cleavage (B).

Fig 6

Lanes: M, molecular mass markers; Lane 1, pellet material after lysate clarification; Lane 2, soluble lysate; Lane 3, StrepTrap column flowthrough; Lane 4, protein washed from column; Lanes 5–11, protein elution fractions; Lane 12, pooled elution fractions; Lane 13; protein after digestion with TEV protease. The expected molecular masses of 8xHis-StrepII-MBP-3607, tag-free 3607 protein, and MBP are 100, 56, and 44 kDa, respectively.