Figure 11.

Gallo 1-Induced Callose Deposition Is Reduced in gae1 gae6 Plants.

(A) Callose deposition in 4-week-old Col-0 and gae1 gae6 plants. Plants were inoculated with B. cinerea isolate Gallo 1 (2.5 × 10⁵ spores mL⁻¹) or mock inoculated with B. cinerea inoculation medium, and samples were collected after 72 h. Callose was stained with aniline blue and visualized using a Nikon Eclipse Ni-U microscope with a 4′,6-diamidino-2-phenylindole filter. Three leaves were stained per treatment. Asterisks indicate fungal hyphae, and arrows indicate representative callose spots. Bars = 100 µm.

(B) Quantification of callose deposition. Callose deposits were counted in 200 × 200-µm squares laid out across the images shown here. Bars represent means ± se from six squares each. Letters indicate significantly different groups (P < 0.05) according to a t test analysis.