Figure 13.

Macerozyme-Induced PTI Is Lost in gae1 gae6 While OG-Induced PTI Is Unaltered.

(A) Macerozyme-induced PTI in Col-0 and gae1 gae6 plants. Four-week-old plants were inoculated with 0.01% macerozyme or mock inoculated with boiled macerozyme and were infected with B. cinerea isolate Gallo 1 (2.5 × 10⁵ spores mL⁻¹) 24 h later. Samples were collected immediately (0 dpi) and after two (2 dpi) and three (3 dpi) days. Data show mean ± se of six to 14 biological replicates each combined using a linear model. Letters indicate significantly different groups (q < 0.05).

(B) OG-induced PTI in Col-0 and gae1 gae6 plants. Four-week-old plants were inoculated with 500 µg mL⁻¹ OGs and infected with B. cinerea as described in (A). Six to eight biological experiments were performed.

(C) and (D) Expression of JAZ5 (C) and JAZ10 (D) was measured in untreated plants or 24 h after inoculation with water (mock), 0.01% macerozyme, or 250 µg mL⁻¹ OGs. Data show mean log₂ ratios to ACTIN2 ± se from three biological replicates combined using a mixed linear model. Letters indicate significantly different groups (q < 0.05). The q-value for comparison of JAZ10 expression in the wild type and mutant after macerozyme treatment is 0.056.