Growth of B. cinerea on gae1 gae6 Plants and the Effect of B. cinerea on Expression of GAE1 and GAE6.
(A) Growth of B. cinerea isolates. Leaves of Col-0 and gae1 gae6 plants were inoculated with 2.5 × 105 spores mL−1 of the B. cinerea isolates indicated in the figure. Samples were collected two (2 dpi) and three (3 dpi) days later. Bars represent means ± se of six biological replicates for each fungus, combined using a mixed linear model. Asterisks indicate significant differences from wild-type Col-0 for each isolate (P < 0.05).
(B) and (C) Expression of GAE1
(B) and GAE6
(C) 48 h after inoculation with the B. cinerea isolates indicated. Expression levels were measured by qRT-PCR. Bars represent mean log2 ratios to ACTIN2 ± se of three biological replicates, combined using a mixed linear model. Letters indicate significantly different groups (q < 0.05).