Truncated Variants of SLAC1 Used in This Study.
(A) Illustration of the full-length SLAC1 protein and its truncated variants. GFP was fused to a series of truncated SLAC1 proteins at the C-terminal region. The amino acid residue numbers are as indicated. A transmembrane region (amino acids 183 to 494) predicted using the ARAMEMNON database (http://aramemnon.uni-koeln.de/) is shown in white.
(B) to (E) Subcellular localization of truncated SLAC1 proteins in the slac1-4 transgenic plants. Fluorescence microscopy images of GFP fused to SLAC1 and its truncated variant proteins, full-length SLAC1 (B), ΔN (C), ΔC (D), and ΔNC (E), in guard cells. Bars = 5 µm.