Figure 6.

Amino Acid Substitutions Did Not Cause Structural Defects.

ABA regulation of relative stomatal aperture in the wild type, the slac1-2 mutant, and transgenic lines expressing mutant SLAC1 protein. Two independent transgenic lines per construct were analyzed. White and gray bars indicate relative stomatal apertures in the absence (control) and presence of 1 µM ABA. Error bars indicate ± se; data from four to five independent experiments were averaged. Asterisks denote comparison with wild-type SLAC1 complementation line: **P < 0.05 by one-way ANOVA with Dunnett's post hoc test.