FIG. 2.

Western blot of uninfected and GAV-infected P. monodon tissues carried out with antiserum to E. coli-expressed GST-ORF2 fusion protein (a) and KLH-linked peptide PN1 (b and c). (a and b) LO (lanes 1 and 2) and gill tissues (lanes 3 and 4) from an uninfected prawn (lanes 1 and 3) and a GAV-infected prawn (lanes 2 and 4). Three proteins smaller than the ORF2 protein detected in LO tissue are indicated (◂). (c) GAV-infected gill tissue (lane 1) and the E. coli-expressed His₆-ORF2 fusion protein (lane 2). Proteins were separated by SDS-PAGE in 12% (a and b) or 15% (c) polyacrylamide gels. The molecular masses (in kilodaltons) of prestained protein standards (Bio-Rad) (a and b) and biotinylated protein standards (Amersham) (c) are indicated (lanes M).