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. 2004 Aug;78(16):8935–8941. doi: 10.1128/JVI.78.16.8935-8941.2004

FIG. 3.

FIG. 3.

Western blot of native ORF2 in GAV-infected LO and gill tissues compared to His6-ORF2 and N-terminally truncated His6-ORF2 fusion proteins expressed in E. coli. (a) GAV-infected gill tissue (lane 1) and LO tissue (lane 6) and IPTG-induced E. coli cultures transformed with pQE-ORF2 (lane 2), pQE-ORF2-M11 (lane 3), pQE-ORF2-M61 (lane 4), and pQE10 (lane 5) were resolved in a 15% polyacrylamide gel and immunodetected with KLH-PN1 peptide antiserum. Lane M contains biotinylated protein standards. (b and c) GAV-infected gill (lane 1) and IPTG-induced E. coli cultures transformed with pQE-ORF2 (lane 2), pQE10 (lane 3), pQE-ORF2-M11 (lane 4), and pQE-ORF2-M61 (lane 5) resolved in a 12% polyacrylamide gel and immunodetected using antiserum to GST-ORF2 (b) or KLH-PN1 peptide (c). Lanes M contain BenchMark prestained protein ladders (Invitrogen). The predominant His6-ORF2 fusion proteins (◂) and minor larger and smaller His6-ORF2 fusion protein forms that were also immunodetected (◃) are indicated.