Figure 5.

Osmotic Water Permeability Coefficient, PIP2;1 Location in X. laevis Oocytes, and Surface pH Analyses.

(A) PIP2;1-G103W- and non-PIP2;1-expressing control water-injected oocytes showed a low water permeability. Results are shown as means ± se measurements from five to eight oocytes from one batch of oocytes.

(B) PIP2;1 and its point mutation isoforms as YFP fusions were present in the vicinity of the oocyte plasma membrane. PIP2;1 alone served as a negative control.

(C) PIP2;1-G103W impaired the CO₂ permeability of PIP2;1 as measured by changes in pH_s. The number of oocytes recorded in the same batch are shown in each bar. Data are mean ± se.