Reduced Levels of HCAAs in the act Mutant.
Coumaroylagmatine (A) and feruloylagmatine (B) levels were determined by UPLC-ESI-QTOF-MS in methanolic extracts of leaves of wild-type and act lines 24 h after inoculation with P. infestans or water. Data are derived from four independent experiments (wild type, water, n = 27; P. infestans, n = 95; act, water, n = 12; P. infestans, n = 23). Error bars represent se. Letters indicate statistically different values (two-way ANOVA, P < 0.05).