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. 2016 Jan 7;28(2):583–596. doi: 10.1105/tpc.15.00706

Figure 4.

Figure 4.

dtx18 Knockout Lines Are Unable to Secrete p-Coumaroylagmatine.

(A) and (B) Characterization of T-DNA insertion lines of DTX18. DNA was isolated from leaves of wild-type plants or the T-DNA insertion lines SALK_062231 and GK_411D06. PCR was performed using specific primers for the alleles from the wild type, SALK_062231 (S), or GK_411D06 (G). C, water control.

(C) Loss of DTX18 gene expression. RT-PCR using DTX18-specific primers was performed using RNA from leaves of wild-type plants and the T-DNA insertion lines SALK_062231 (S) and GK_411D06 (G). Genomic DNA (g) was used as a control.

(D) and (E) Reduced extracellular levels of coumaroylagmatine in dtx18 mutant lines. Coumaroylagmatine levels were determined in leaves (D) and in droplets of the inoculum (E) from Arabidopsis wild-type and mutant plants (SALK_062231 and GK_411D06) 24 h after inoculation with P. infestans. Data shown were obtained in at least three independent experiments (wild type, n ≥ 106; act, n = 21; SALK_062231, n = 47; GK_411D06, n = 8). Significance analysis of differences was performed by t test, *P < 0.05, **P < 0.01, and ***P < 0.001. fw, fresh weight.