Figure 5.

Expression of ACT and DTX18 in Transgenic Potato Plants.

(A) and (B) qRT-PCR analysis for ACT (black bars) or DTX18 expression (gray bars) was performed with RNA from leaves of wild-type and transgenic potato plants carrying an empty vector construct (ev) or expressing 35S-ACT (lines B, H, M, and Z) (A) or 35S-ACT-35S-DTX18 (lines D, K, N, and X) (B). Expression of EF1a was used as a reference. Data are derived from two independent experiments ([A]: wild type, n = 7; empty vector, n = 5; B, M, H, and Z, n = 3; [B]: wild type, n = 8; empty vector, n = 6; D, K, N, and X, n = 3). Error bars represent se. nd, not detectable.

(C) Phenotype of 35S-ACT-35S-DTX18 potato plants.