Characterization of ACT- and ACT-DTX18-Expressing Potato Plants.
Coumaroylagmatine levels were determined in leaves ([A] and [B]) and in droplets of the inoculum ([C] and [D]) from wild-type and transgenic potato plants carrying an empty vector construct (ev) or 35S-ACT (lines B, H, M, and Z) ([A] and [C]) or 35S-ACT-35S-DTX18 (lines D, K, N, and X) ([B] and [D]). Significance analysis of differences was performed by t test, *P < 0.05, **P < 0.01, and ***P < 0.001.
(A) Two independent experiments; wild type, empty vector, n = 10; B, n = 5; H, M, and Z, n = 6.
(B) Three independent experiments; wild type, empty vector, n = 18; D2, n = 7; K3, n = 6; N3, n = 7; X2, n = 8.
(C) Two independent experiments; wild type, empty vector, n = 10; lines B, H, M, and Z, n = 6.
(D) Three independent experiments; wild type, empty vector, n = 18; D2, n = 7; K3, n = 5; N3, n = 6; X2, n = 7.