Figure 4.

The Arabidopsis LSM1-7 Complex Interacts with Selected Target Transcripts in Response to Abiotic Stresses.

RIP assays of 2-week-old c-lsm1a plants grown under control conditions: control exposed for 24 h to 4°C (A), 10 h to 55% PEG (B), or 10 h to 150 mM NaCl (C), using an anti-GFP antibody. RIP assays of Arabidopsis containing a GRP7_PRO-GRP7-GFP fusion grown under control and stress conditions were also performed as interaction specificity controls. Co-IP RNA samples corresponding to different cold- (A), drought- (B), and salt-inducible (C) genes were quantified by qPCR. Transcript levels in c-lsm1a plants were corrected with respect to their corresponding input values and are represented relative to the levels obtained from RIP control assays. In all graphs, error bars indicate the sd of the mean (n ≥ 3). Asterisks indicate significant differences (*P ≤ 0.01, **P ≤ 0.001, and ***P ≤ 0.0001) in transcript levels between RIP assays from stressed and control plants, as determined by t test.