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PTGS and siRNA Accumulation in Double Mutants Involving smd1b.
(A) Percentages of plants silenced by PTGS in the indicated genotypes determined by quantitative GUS activity measurements (n = 96 plants for each genotype).
(B) RNA gel blot analyses of GUS siRNAs in the indicated genotypes. U6 snRNA hybridization served as a loading control. Note that the sm1d upf1 double mutant does not contain the 159 locus because upf1 and 159 loci are very close.
