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. 2016 Mar 14;11(3):e0151126. doi: 10.1371/journal.pone.0151126

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid Genotype or relevant features Reference or source
Salmonella Typhimurium
LB5010 a LT2 strain derivative, Fim+ and fimbrial phase variable [23]
Salmonella Choleraesuis
SC-15, 27, 30, 46, 77 Clinical isolate, Fim- This study
SC-31, 34, 39 Clinical isolate, Fim+ and fimbrial phase variable This study
Escherichia coli
DH5α [24]
Plasmids
yT&A 2.7 kb cloning vector that contains T7 promoter; Amr Yeastern, Taiwan
pACYC184 4.2 kb cloning vector; Tetr and Cmr ATCC
pISF101 12.8 kb DNA with the complete fim genes of S. Typhimurium cloned into pACYC184 [25]
pfimAICDHF 8.0 kb fimAICDHF DNA fragment cloned into pACYC184; Cmr This study
pfimZY0551WU 3.7 kb fimZY0551WU DNA fragment cloned into pACYC184; Cmr This study
pfimAI 1.4 kb fimAI DNA fragment cloned into pACYC184; Cmr This study
pfimAICD 6.1 kb fimAICD fragment cloned into pACYC184; Cmr This study
pfimHF 1.6 kb fimHF fragment cloned into pACYC184; Cmr This study
pfimH 1.2 kb fimH DNA fragment cloned into pACYC184; Cmr This study
pfimHL57P A fimH coding sequence with L57P cloned into pACYC184; Cmr This study
pfimHG63V A fimH coding sequence with G63V cloned into pACYC184; Cmr This study
pfimHR89Q A fimH coding sequence with R89Q cloned into pACYC184; Cmr This study
pfimHA115T A fimH coding sequence with A115T cloned into pACYC184; Cmr This study
pfimHS131Y A fimH coding sequence with S131Y cloned into pACYC184; Cmr This study
pfimHG177S A fimH coding sequence with G177S cloned into pACYC184; Cmr This study