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. 2016 Mar 14;11(3):e0151397. doi: 10.1371/journal.pone.0151397

Fig 4. Ori increases the expression of PSD-95 and synaptophysin and promotes mitochondrial activity in the synaptosomes of AD mice.

Fig 4

(A) Western blotting for PSD-95, synaptophysin, COX-2 and Lamin B1 proteins. The absence of the cytosolic protein COX-2 and the nuclear protein Lamin B in the synaptosome pellets confirms the purity of synaptosomes. (B) Quantitative analysis of PSD-95 and synaptophysin expression. The relative expression levels of PSD-95 and synaptophysin were normalized against β-actin and are presented as the ratio of the values of the experimental group to the control group. (C) Western blotting for VDAC1 protein. (D) Quantitative analysis of VDAC1 expression. (E) MTT assay for mitochondrial activity of synaptosomes. n = 5 mice per group and the experiment was performed for three times. Data are presented as the means ± SEM. *P<0.05, **P<0.01 vs. control; #P<0.05, ##P<0.01 vs. Aβ1–42.