FIG. 3.
Effect of reintroduction of DNA-PKCS into the scid background on transduction efficiency by an HIV-1 vector. ST.SCID and 100E cells were distributed in a 96-well plate at a density of 104 cells per well. Cells were infected 24 h later with undiluted virus (MOI of ≈0.01) or the indicated dilutions of the HIV-1 lacZ vector preparations as described in the legend to Fig. 1. Two days postinfection, cells were stained using a β-galactosidase assay (Stratagene), blue cells were counted (two wells/datum point), and digital micrographs were taken of cells infected with the undiluted virus. ST.SCID cells (black columns) and 100E cells (shaded columns) are shown.