Table 1. List of primers used in this study.
Primer name | Sequence (5’-3’) | Reference |
---|---|---|
PPV2NotI | GATTAGCGGCCGCCATGAGCGCTGCCGA | This study |
PPV2PacI | CGGTTAATTAATTATACACGATGAGCGCGT | This study |
PPV2AF | ACACGATGAGCGGTACGA | [4] |
PPV2AR | TCCTCACGAGGTCTCTTCTG | [4] |
PPV2-EcoRI-2035F | GGAATTCTTTCCCAGTCTCGAACC | This study |
PPV2-XhoI-2826R | GGCTCGAGCACCTGCGGCTGCAT | This study |
Enzyme cleavage sites are underlined.