Skip to main content
. 2016 Mar 14;11(3):e0151416. doi: 10.1371/journal.pone.0151416

Fig 5. Impact of inhibiting mTOR on inflammation in endometrial tissue.

Fig 5

Ex vivo organ cultures of endometrium were cultured for 24 h in medium containing 1.8 mg/organ glucose with vehicle (0), or rapamycin (a-c: 250, 500, 1000 nM) or Torin 1 (d-f: 250, 500, 1000 nM). Media was then aspirated and replenished with fresh medium containing a corresponding concentration of glucose and rapamycin or Torin 1, and challenged with control vehicle or 100 ng/ml LPS for a further 24 h. At the end of the experiment, organ weights were recorded, and the accumulation of IL-1β (a, d), IL-6 (b, e) and IL-8 (c, f) was measured in supernatants. Data are presented as mean concentration per mg tissue + SEM from 4 independent experiments, and analyzed by ANOVA using Dunnett’s multiple comparisons test to compare with vehicle (0), within each treatment group; * P < 0.05, ** P < 0.01, *** P < 0.001, NS = ANOVA not significant.