Fig 9. Cytokine in the supernatants of CD4⁺ T cells and MoDCs stimulated by SC19.

CD4⁺ T cells were mixed with 5-day old MoDCs in the proportion of 4:1. The mixed cells were stimulated by SC19 (MOI = 0.1), LPS (1 μg/mL) or Pam3CSK4 (500 ng/mL) at 37°C with 5% CO₂. 17 hours later, supernatant from each well were collected and analyzed for IL-4, IL-17, IL-12p70, and IFN-γ using Human Th1/Th2/Th17 Antibody Assay.