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Localization of HELLP-RFP in homokaryotic mycelium and localization of HELLP-RFP point mutants. (A) HELLP-RFP is expressed in a wild-type strain and shows a diffuse cytoplasmic localization. (Scale bar: 5 μ.) (B) HELLP-RFP G9I and G13I mutants do not produce a barrage reaction to HELLP(215-278)-GFP, whereas wild-type HELLP-RFP does (white arrowhead). (C) Localization of HELLP-RFP G9I and HELLP-RFP G13I in fusion cells with strains expressing HELLP(215-278)-GFP. (Scale bar: 5 μ.)
