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. 2016 Feb 22;113(10):2696–2701. doi: 10.1073/pnas.1518240113

Fig. S7.

Fig. S7.

P. alecto recombinant IFN expression. Conditioned media from HEK293T cells transfected with plasmids encoding pCAGGS-GFP (GFP control, lane 1), pcDNA6.2/C-emGFP–IFN-α1 (lane 2), -IFN-α2 (lane 3), or -IFN-β (lane 5) were evaluated by immunoblotting with anti-GFP antibody. Conditioned media from HEK293T cells transfected with pCAGGS/FLAG-IFN-α3 plasmid (lane 6) or mock-transfected (lane 7) were evaluated by using anti-FLAG antibody. The molecular weight marker (lane 4) is shown with additional labeling in kilodaltons on the right. The approximate molecular weights of the proteins are as follows: GFP, 27 kDa; IFN-α1–GFP, 46 kDa; IFN-α2–GFP, 46 kDa; IFN-α3–FLAG, 23 kDa (migrating as a doublet as a result of posttranslational modification); and IFN-β-GFP, 46 kDa.