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. 2016 Feb 22;113(10):E1382–E1391. doi: 10.1073/pnas.1525586113

Fig. 3.

Fig. 3.

Presynaptic PLC and PKC activities are necessary for DOI-elicited potentiation of NMDA currents. (A) Preincubation of slices for 10 min with the PKC inhibitor NPC15437 (20 μM, n = 9) or the PLC inhibitor U73122 (5 μM, n = 10) prevents potentiation of NMDA EPSCs induced by DOI. Note that the top graph is the same graph as that illustrated on Fig. 1A. (B) Loading recorded layer V neurons with the G protein inhibitor GDPβS (1 mM, n = 16) or with U73122 (5 μM, n = 9) has no effect on the potentiation of NMDA currents, whereas intracellular perfusion of the PKC inhibitor NPC15437 (20 μM, n = 10), or the calcium chelator BAPTA (20 μM, n = 12), inhibits the late phase of potentiation. Plots showing the time course of NMDA EPSCs in response to the treatments are shown (Left). The histograms show the normalized peak amplitude of NMDA EPSCs. (Right) Representative traces of NMDA EPSCs before (gray) and after (black) DOI application. *P < 0.05; **P < 0.01; ***P < 0.001 vs. baseline (Bl); n.s., not significant (Student’s t test or one-way ANOVA).