Fig. S4.
Related to Fig. 2. 5-HT2A-IRES-GFP viral rescue experiments. (A and B) GFP fluorescence in mPFC (A) and MDN (B) 1 d after 5-HT2A-IRES-GFP virus injection. (Magnification: A and B, 10×.) (C) Expression pattern of recombinant 5-HT2A receptors. (Top) immunofluorescence labeling of recombinant 5-HT2A receptors in PFC (red signal) shows colocalization of 5-HT2A receptor immunoreactive signal (red) with the GFP signal (green signal, arrowheads). (Scale bar, 30 μm.) (Middle) Virally infected neurons (GFP+ cells) show NeuN immunostaining (pyramidal neurons, red signal). (Scale bar, 15 μm.) (Bottom) Virally infected neurons do not show parvalbumin (PV) immunostaining. (Scale bar, 50 μm.)