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. 2016 Feb 22;113(10):E1382–E1391. doi: 10.1073/pnas.1525586113

Fig. S5.

Fig. S5.

Related to Fig. 4. Ifenprodil perfusion does not modify NMDA transmission at thalamocortical synapses. (A) After a period of baseline (Bl), ifenprodil (Ifen., 3 μM) was perfused in the bath (n = 6). The histogram shows the quantification of NMDA EPSCs, normalized to values obtained in baseline condition (Bl, gray line), following 12 min of ifenprodil application (black line). (Right) Representative traces of NMDA EPSCs. Student’s t test. (B) PFC slices from 5-HT2A−/− mice were treated with either vehicle or DOI (1 μM) for 20 min. GluN2B phosphorylation at Ser1303 was assessed by sequential immunoblotting with the phospho-site specific antibody and the antibody recognizing GluN2B independently of its phosphorylation state. Representative Western blots are illustrated. Data, expressed in percent of baseline, represent ratios of phosphorylated to total GluN2B. They are the means ± SEM of three independent experiments.