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Sequence alignments of YchFs. Sequences of OsYchF1, human OLA1 (hOLA1), and YchFs from T. thermophilus (ThYchF), S. pombe (SpYchF), and H. influenzae (HiYchF) were aligned by using the program MUSCLE (36). The G1–G5 motifs and domain boundaries are indicated below the alignment. The secondary structural elements are numbered according to the convention used in HiYchF (6) and hOLA1 (4). Note: Helix α3 is unfolded in GMPPNP-OsYchF1 and in apo-OsYchF1 at pH 6.5.
