Fig. 1.

Growth and O₂ evolution of the cgl71 mutant. (A) WT, cgl71, and cgl71(CGL71) rescued cells were grown at 30 µmol photons⋅m⁻²⋅s⁻¹ (µE) in liquid TAP medium and then were washed and spotted onto solid TAP medium and allowed to grow for 5 d at various light intensities, as indicated at the top of the image. (B) Rate of O₂ evolution of WT and cgl71 mutant cells after growth at 30 µE. Cells were pelleted by centrifugation (3,200 × g) for 10 min, resuspended in 50 mM Hepes (pH 7.5) containing 5 mM NaHCO₃, and shaken in the dark for 30 min. During the assay, samples were illuminated at increasing light intensities, as indicated on the x axis, for 2 min each, followed by a 2-min dark incubation. The rate of O₂ evolution at each light intensity was corrected for the rate of dark respiration. Each point represents the mean of three biological replicates.