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. 2016 Feb 22;113(10):2774–2779. doi: 10.1073/pnas.1524040113

Fig. 4.

Fig. 4.

Changes in PSI during hypoxic growth. Strains were grown under LO conditions for 5 d before being moved to DO or DH conditions for 4 d beginning at day 0. (A) Level of oxidizable P700 relative to chl for WT and cgl71 cells maintained in DO and DH conditions. Points are average values from three saturating flashes. All traces were corrected for potentially interfering absorbance at 740 nm. The results shown represent the average of three biological replicates. (B) Immunological detection of specific thylakoid proteins from WT and cgl71 cells. Proteins extracted from cells containing 1 μg of chl were resolved by SDS/PAGE (15% polyacrylamide gel) and were detected immunologically using monospecific antibodies raised to the polypeptides indicated at the right of the figure. Growth conditions are given at the top of the immunoblot. All protein designations are as in Fig. 2.