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. 2016 Feb 22;113(10):2774–2779. doi: 10.1073/pnas.1524040113

Fig. S2.

Fig. S2.

Analysis of two tetrads (WT crossed to cgl71) for Zeocin resistance (ble) and growth on HS and TAP medium. (A) The cells were spotted on agar plates and grown for 10 d at 30 µmol photons⋅m−2⋅s−1. The Zeocin-supplemented medium contained 5 μg/mL Zeocin. Strains 1–4, progeny from tetrad #1; strains 5–8, progeny from tetrad #2; strain 9, WT; strain 10, cgl71; lane 11, H2O control (no template) only for B–D. (B) PCR using RMD264/CGL71-R2 primers to detect the ble insertion. (C) PCR using CGL71-F2/CGL71-R2 primers to detect the intact CGL71 gene. (D) PCR using mating-type primers. The upper bands in D represent mating type minus, which is 600 bp. The lower bands in D represent mating type plus, which is 500 bp.