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. 2016 Feb 22;113(10):2774–2779. doi: 10.1073/pnas.1524040113

Fig. S6.

Fig. S6.

PSI turnover under DO conditions (in the presence of chloramphenicol). Strains were grown under LO conditions. They then were treated with chloramphenicol (CAP) (final concentration, 250 μg/mL) and were moved to DO conditions for 3 d beginning on day 0. (A) Level of oxidizable P700 relative to chl for WT and cgl71 cells maintained in CAP-DO conditions (normalized to day 0). The results shown are the average of three biological replicates. (B) Immunological detection of specific thylakoid proteins from WT and cgl71 cells. Proteins extracted from cells containing 1 μg of chl were resolved by SDS/PAGE (15% polyacrylamide gel) and quantified by immunoblot analysis using monospecific antibodies raised to the polypeptides (indicated at the right of the figure). Times following movement of the cells to DO conditions are indicated at the top of the immunoblot. Triple asterisks indicate that three times more cgl71 sample (based on chl) than WT sample was resolved on the gel to achieve a roughly even load of PSI subunit polypeptides at day 0.