Fig. S7.

Mating-based split-ubiquitin system showing no protein–protein interactions of CGL71 and PSI subunits (PsaC, PSAD, and PSAE). (A) Yeast diploids containing two vectors (indicated above and at the left) were grown on both permissive (Upper) and restrictive (Lower) plates (see SI Materials and Methods, Mating-Based Split-Ubiquitin Assay). (B) The NubG (empty) vector combined with CGL71-Cub or Cub served as negative controls, and the NubWT vector combined with CGL71-Cub or FAD6-Cub vector combined with FDX5-Nub served as positive controls (51). Each interaction was confirmed by colony PCR. Three different colonies were picked for the assay, which showed similar results in the three colonies.