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UTRs have no significant role in regulating TLR7 protein expression. TLR7 constructs with (+) or without (−) endogenous UTRs were stably integrated into HEK293 Flp-In T-REx cells. After 18 h of doxycycline treatment, cells were lysed and analyzed by SDS/PAGE Western blot with the indicated antibodies. Data are representative of at least three independent experiments.
