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. 2016 Jan 25;113(10):2738–2743. doi: 10.1073/pnas.1520376113

Fig. 5.

Fig. 5.

Highly coexpressed genes are potential steroid targets. (A) Coronal ISH sections showing the expression of Esr1, Irs4, and Magel2. (Scale bars, 600 μm.) Data taken from the Allen Brain Atlas (4). Response of Irs4 (B) and Magel2 (C) to DES treatment in castrated mice in the MPO and ARH using dISH. (D) dISH of Esr1 (red) and Irs4 (green) in the anterior HY. (Scale bars, 10 μm. Magnification, 100×.) (E) dISH of Esr1 (red) and Magel2 (green) in the anterior HY. (Scale bars, 10 μm. Magnification, 100×.) mRNA expression in ISH was quantified as the percentage of the image surface with positive signal. Reported P values are calculated with a one-sided, two-sample t test with a significant level at P < 0.05.