. Author manuscript; available in PMC: 2016 Mar 14.

Published in final edited form as: Environ Sci Technol. 2015 May 21;49(23):13749–13759. doi: 10.1021/es506358x

Table 3.

Summary of the CHO Cell Acute Genotoxicity of the Target HALs

		concn range (µM)	lowest %TDNA genotoxic concn (µM)^a	50% TDNA (µM)^b	R²^c	ANOVA test statistic^d
Mono-HALs and di-HALs	CAL	50–500	100	142.8	0.99	F_10,59 = 62.6; P ≤ 0.001
	BAL	100–550	200	381.2	0.98	F_10,68 = 57.2; P ≤ 0.001
	IAL	100–1000	900	1009	0.98	F_13,103 = 22.5; P ≤ 0.001
	DCAL	50–2000	800	795	0.98	F_19,60 = 64.0; P ≤ 0.001
	DBAL	50–300	50	111.3	0.98	F_9,44 = 41.5; P ≤ 0.001
	BCAL	100–700	500	621.4	0.92	F_10,51 = 22.0; P ≤ 0.001
tri-HALs	TCAL	50–5000	NS^e	NS^e	NS^e	F_20,37 = 0.556; P = 0.918
	TBAL	25–500	100	340.3	0.99	F_11,64 = 168; P ≤ 0.001
	BDCAL	60–600	300	470.4	0.91	F_17,106 = 16.4; P ≤ 0.001
	DBCAL	60–220	100	143.7	0.99	F_5,29 = 34.4; P ≤ 0.001

The lowest genotoxic concentration was the lowest concentration of the haloacetaldehyde in the concentration–response curve that induced a statistically significant amount of genomic DNA damage as compared to the negative control.

The SCGE 50% Tail DNA value is the haloacetaldehyde concentration determined from a regression analyses of the data that was calculated to induce, on average, 50% of the genomic DNA of the nucleoids to migrate into the gel.

R² is the coefficient of determination for the regression analysis upon which the SCGE % Tail DNA value was calculated.

The degrees of freedom for the between-groups and residual associated with the calculated F-test result and the resulting probability value.

NS = not significantly different from the negative control.