Table 3.
Serial Dilution Experiments for Mutation Detection With MitoChip
| RCRS base # | Sequence in lymphocyte DNA | Sequence in pure tumor DNA | Sequence in 1:50 diluted tumor DNA |
|---|---|---|---|
| 750 | G | A | A + G |
| 1007 | G | A | A + G |
| 2000 | C | T | C + T |
| 2352 | T | C | C + T |
| 4655 | G | A | A + G |
| 5262 | G | A | A + G |
| 5421 | G | A + G | No calla |
| 6524 | T | C | No calla |
| 8701 | A | G | A + G |
| 9540 | T | C | C + T |
| 9547 | G | G + T | No call |
| 10398 | A | G | A + G |
| 10873 | T | C | No calla |
| 11180 | G | A + G | A + G |
| 11350 | A | G | A + G |
| 12248 | A | G | A + G |
| 12705 | C | T | C + T |
| 13101 | A | C | A + C |
| 13197 | C | T | C + T |
| 13650 | C | C + G | No call |
| 14212 | T | C | C + T |
| 14766 | C | T | C + T |
| 15812 | G | A | A + G |
| 15904 | T | C | C + T |
a
Heterozygous call is present at these three nucleotide positions (5421 = A + G; 6524 = C + T; 10873 = C + T) in the 10-fold diluted tumor DNA sample