Table 2.
List of PCR primers used for Frankia strain genotyping
| Gene | Primer name1 | Sequence (5′→3′) | Tm (°C) |
|---|---|---|---|
| Field nodule identification (infectivity experiments) | |||
| dnaA | dnAfdT7F | TAATACGACTCACTATAGGGGAGGARTTCACCAACGACTTCAT | 62 |
| dnArvT3R | ATTAACCCTCACTAAAGGGACRGAAGTGCTGGCCGATCTT | ||
| pgk | pgkFwdT3 | ATTAACCCTCACTAAAGGGATGAGGACGATCGACGACCTGC | 62 |
| pgkRevT7 | TAATACGACTCACTATAGGGCGCSAGGAAGGTGAAGCACAT | ||
|
| |||
| Sp+/Sp− discrimination in nodules (co-inoculation experiments) | |||
| dnaA | F2 Sp− | CCATGGAGACGCCGAAGTAC (1341)2 | 65 |
| F3 Sp− | CGTCCGGGATCAGGTCG (1275) | 64–65 | |
| R2 Sp− | CATCGCGATCCTGTCGAAGAAG (1089) | 65 | |
| F3 Sp+ | GCGTCAGGGATCAGGTCA (1275) | 64 | |
| R2 Sp+ | CATCGCGATCCTGTCGAAAAAA (1089) | 64 | |
1
“fd”, “Fwd”, or “F” in the name indicate forward primers and “rv”, “Rev”, or “R” indicate reverse primers.
2
Position on the dnaA sequence of the ACN14a strain (GenBank accession NC_008278).