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Comparison of chemolithoautotrophic growth (A) and symbiotic phenotypes (BC) between hup mutants and the wild-type strain of B. diazoefficiens USDA110. (A) Growth phenotype on Hup agar medium under an atmosphere of 84% N2, 10% H2, 5% CO2, and 1% O2 at 25°C. (B) Plant growth 30 d after inoculation. (C) Concentration of H2 produced by the root nodules. White squares, wild type; black squares, ΔhupS1L1 mutant; grey squares, ΔhupS2L2 mutant.
