Table 1.
Strains and plasmids used in the present study.
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Bradyrhizobium diazoefficiens | ||
| USDA110 | Soybean bradyrhizobia, wild type | 11 |
| ΔhupS1L1 | USDA110 hupS1L1::aadA; Smr Spr | This study |
| ΔhupS2L2 | USDA110 hupS2L2::tet; Tcr | This study |
| Escherichia coli | ||
| DH5α | cloning strain | Toyobo Inc.a |
| Plasmids | ||
| p34S-Tc | Plasmid carrying a 2.1-kb Tc cassette; Tcr | 2 |
| pHP45Ω | Plasmid carrying a 2.1-kb Ω cassette; Spr Smr Apr | 15 |
| pK18mob-hupI | pK18mob carrying a 5.9-kb hupFDCL1S1VU HindIII fragment of brp15657; Kmr | This study |
| pK18mob-hupIad | pK18mob-hupI with an ApaI/EcoRI adaptor; Kmr | This study |
| pK18mob-hupIomega | pK18mob carrying hupS1L1::aadA; Smr Spr Kmr | This study |
| pK18mob-hupII | pK18mob carrying a 7.6-kb hupS2L2CDFHK XhoI fragment of brp07428; Tcr | This study |
| pK18mob-hupIIad | pK18mob-hupII with an EcoO109I/BamHI adaptor; Kmr | This study |
| pK18mob-hupIITc | pK18mob carrying hupS2L2::tet; Tcr Kmr | This study |
| pK18mob | cloning vector; pMB1ori oriT; Kmr | 16 |
| pRK2013 | ColE1 replicon carrying RK2 transfer genes; Kmr | 5 |
| brp15657 | pUC18 carrying hupUVS1L1CDFG | 11 |
| brp07423 | pUC18 carrying hupKHFDCL2S2 | 11 |
Apr, ampicillin-resistant; Tcr, tetracycline-resistant; Kmr, kanamycin-resistant; Smr, streptomycin-resistant; Spr, spectinomycin-resistant.
a
Osaka, Japan.