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. 2016 Mar 15;27(6):1026–1039. doi: 10.1091/mbc.E15-07-0521

TABLE 3:

Primers used for site-directed mutagenesis.

Description Primer sequence
ERK2-T188A-F 5′-GGGTTCTTGACAGAGTATGTAGCCGCGCGTTGGTACAGAGC-3′
ERK2-T188A-R 5′-TGTACCAACGCGCGGCTACATACTCTGTCAAGAACCC-3′
ERK2-T188D-F 5′-GGGTTCTTGACAGAGTATGTAGCCGACCGTTGGTACAGAGC-3′
ERK2-T188D-R 5′-TGTACCAACGGTCGGCTACATACTCTGTCAAGAACCC-3′
MPK1-K54R-F 5′-CCACAGTTGCCATCAGGAAAGTGAC-3′
MPK1-K54R-R 5′-GTCACTTTCCTGATGGCAACTGTGG-3′