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. 2016 Mar 15;27(6):954–966. doi: 10.1091/mbc.E15-06-0417

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© 2016 Fujiwara et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Knockdown of Solo disorganizes actin and keratin cytoskeletons. (A) Confocal microscopic images of K8 filaments and F-actin in control and Solo-knockdown cells. MDCK/YFP-K8 cells were transfected with control or Solo-targeting siRNAs, cultured for 48 h, fixed, and stained with Alexa Fluor 568–phalloidin. The dashed line indicates the cell outline. Scale bars, 20 μm. (B) Effects of Solo knockdown on expression of Solo. MDCK cells were transfected with control or Solo-targeting siRNAs and cultured for 48 h. Cell lysates were analyzed by anti-Solo immunoblotting. (C) Effect of Solo knockdown on the organization of K8 networks. Percentages of cells with organized K8 networks. (D, E) Effect of Solo knockdown on the thickness of K8 fibers. The diameter of the thickest K8 fiber in each cell was measured, and the data are displayed as histograms (D) or dot plots (E). (F) Effect of Solo knockdown on stress fiber formation. Percentages of cells with thick, moderate, or thin stress fibers. In C and F, the data represent the mean ± SD of three independent experiments (at least 10 cells per experiment). **p < 0.01 (one-way ANOVA followed by Dunnett’s test).