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. 2016 Mar 15;27(6):954–966. doi: 10.1091/mbc.E15-06-0417

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© 2016 Fujiwara et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Effects of Y-27632 on actin and keratin organization. (A) Confocal microscopy of YFP-K8 and F-actin. MDCK/YFP-K8 cells were treated with 10 μM Y-27632 or left untreated for 1 h and then fixed and stained with Alexa Fluor 568–phalloidin. The dashed line indicates the cell outline. Scale bars, 20 μm. (B, C) Effects of Solo knockdown on the organization of K8 networks (B) and stress fiber formation (C), analyzed as in Figure 2. The data represent the mean ± SD of three independent experiments (at least 21 cells/experiment). **p < 0.01 (two-tailed paired t test).