Table 2.

Regenerated tissue NO concentration (×10⁻³ µM/µg)

Time (min)	Mature sham	Mature loaded	Aged sham	Aged loaded
15	18.4 ± 15.0	29.2 ± 20.9	0.4 ± 0. 5**	1.6 ± 3.5***
30	12.4 ± 7.9	30.5 ± 26.0	0.1 ± 0.3**	1.1 ± 2.5***
45	28.2 ± 27.4	42.9 ± 30.5	4.9 ± 5.7*	0.7 ± 1.6***
60	37.2 ± 33.7	36.6 ± 22.0	6.2 ± 8.9	14.6 ± 22.1

Values are expressed as mean ± SD (n = 11 sham, n = 11 loaded for mature rats and n = 9 sham, n = 9 loaded for aged rats). One extreme outlier NO sample was removed from every group except aged 15 min sham, mature 15 min sham, mature 30 min sham, and mature 60 min sham

^*p < 0.05 between sham-treated explants from aged animals and loaded explants from mature animals,

^**p < 0.01 between sham-treated explants from aged animals and loaded explants from mature animals, and

^***p < 0.01 between age groups for loaded explants